Bioinformatics Services

Contract research organizations (CROs) offer comprehensive functional genomics laboratory services. Such laboratory services often depends on bioinformatic analysis of the data.

As further development of functional genomics applications and associated laboratory services a number of bioinformatics software applications were developed for gene and genome sequencing applications, genetic and genomic data analysis, high through data analysis, whole-genome library screening experiments, etc. For example, Sanger sequencing has been utilized for conventional sequencing applications as well as to evaluate positive RNAi screening results, while the microarray hybridization assays are commonly used as a means of evaluating large expression data sets and negative selection screens.

These methods are relatively costly and time consuming, therefore an alternative Next Generation Sequencing (NGS) has been recently developed. Using massive parallel sequencing provide advantage both in terms of range and input library flexibility, as well as scaling up applications.

Bioinformatics Services (Company):

Forensic Bioinformatics – Link

NCBI Genbank – Link

EMBL-EBI Tools, Services, Datatases – Link

Q2 Genomic Testing and Expression Analysis – Link

Sequentix Bioinformatics Services – Link

Craic Computing – Link

BaseClear Bioinformatic Services – Link

 

News:

Nature Cell Biology - Issue - nature.com science feeds

Articles and research papers on cell division, cell structure, animal and plant cell biology and cell cycles.

The metabolic transition from mitochondrial oxidative phosphorylation (OXPHOS) to glycolysis is critical for somatic reprogramming of induced pluripotent stem cells (iPSCs). SIRT2 has now been established as a previously unknown regulator of this metabolic transition during somatic reprogramming by controlling the acetylation status of glycolytic enzymes.
Author: Tong Ming Liu
Posted: April 27, 2017, 12:00 am
The role of the epithelial-to-mesenchymal transition in tumour progression remains a topic of intense debate. Now, data on the role of Zeb1 in the metastatic spread of pancreatic cancer clarify apparently conflicting views by revealing context-specific, differential use of individual epithelial-to-mesenchymal transition transcription factors in cancer cell dissemination.
Author: M. Angela Nieto
Posted: April 27, 2017, 12:00 am
A feature of the cell cycle is that the events of one cycle must be reset before the next one begins. A study now shows that the replication machinery is removed from fully replicated DNA by a conserved ubiquitin- and CDC48 (also known as p97)-dependent pathway. This explains how eukaryotic chromosomes are returned to the unreplicated state.
Author: Vincent Gaggioli
Posted: April 27, 2017, 12:00 am
Dysfunctional cells are eliminated from epithelial monolayers by a process known as cell extrusion to maintain tissue homeostasis. Normal epithelial cells are now shown to induce the extrusion of oncogene-transformed cells by inducing metabolic changes in the oncogene-expressing cells through PDK4-mediated inhibition of PDH and mitochondrial metabolism.
Author: Jonathan L. Coloff
Posted: April 27, 2017, 12:00 am
Saade et al. show that Shh activation promotes symmetric pericentrin-mediated docking of PKA to centrosomes and the expansion of the motor neuron progenitor pool through symmetric proliferative divisions.
Author: Murielle Saade
Posted: April 27, 2017, 12:00 am
The steep cuts in science funding proposed in the 2018 US budget blueprint have raised alarm in scientific quarters, and signal the current administration's disregard for the significance of science and research in modern society.
Posted: April 27, 2017, 12:00 am
Jiang et al. show that the microcephaly-associated protein ASPM and katanin form a complex that binds microtubule minus ends and can sever microtubules and block microtubule minus-end elongation to control spindle pole dynamics.
Author: Kai Jiang
Posted: April 24, 2017, 12:00 am
Sah and colleagues show that the volume-sensitive ion channel SWELL1 regulates adipocyte insulin-PI(3)K-AKT2 signalling, glucose uptake and lipid content through interactions with GRB2/Cav1.
Author: Yanhui Zhang
Posted: April 24, 2017, 12:00 am
Cha et al. show that SIRT2 suppression by miR-200c enhances acetylation levels and enzymatic activities of glycolytic enzymes and contributes to metabolic reprogramming of human induced pluripotent stem cells and human embryonic stem cells.
Author: Young Cha
Posted: April 24, 2017, 12:00 am
Chen et al. generate lung bud organoids from human pluripotent stem cells that recapitulate early lung development, such as branching airway formation and early alveolar structures, which could potentially be used to model lung disease.
Author: Ya-Wen Chen
Posted: April 24, 2017, 12:00 am
Lin and Wang show that methionine deprivation reprogrammes bacterial metabolism to regulate host mitochondrial dynamics and lipid metabolism in Caenorhabditis elegans through nuclear receptor and Hedgehog signalling.
Author: Chih-Chun Janet Lin
Posted: April 24, 2017, 12:00 am
The non-essential amino acids serine and glycine are critical for proliferative metabolism. A study in Nature now finds that dietary serine and glycine deprivation inhibits growth of some tumours. Whether this dietary intervention is effective depends on both the oncogenic context and tumour tissue of origin.
Author: Mark R. Sullivan
Posted: April 19, 2017, 12:00 am
Adding to the recent debate on the role of epithelial–mesenchymal transition (EMT) in cancer cell invasion and metastasis, Brabletz and colleagues show that the EMT-inducing transcription factor Zeb1 drives pancreatic tumorigenesis and metastasis.
Author: Angela M. Krebs
Posted: April 17, 2017, 12:00 am
Porpiglia et al. use single-cell mass cytometry to analyse surface markers and key myogenic transcription factors of skeletal muscle stem cells during homeostasis and repair, and identify previously unrecognized myogenic progenitor cell populations.
Author: Ermelinda Porpiglia
Posted: April 17, 2017, 12:00 am
Reilein et al. demonstrate that Drosophila ovarian follicle stem cells (FSCs) produce either proliferative follicle cells or quiescent escort cells, depending on which position they occupy in the germarium and in response to graded Wnt signalling.
Author: Amy Reilein
Posted: April 17, 2017, 12:00 am
Fujita et al. find that normal epithelial cells induce metabolic changes in adjacent transformed cells, causing their apical extrusion. The effect is conveyed non-cell-autonomously and relies on PDK4-mediated inhibition of mitochondrial function.
Author: Shunsuke Kon
Posted: April 17, 2017, 12:00 am
Damm et al. demonstrate that trunk neural crest cell migration to the dorsal aorta in zebrafish is regulated by platelet-derived growth factor (PDGF) signalling and required for haematopoietic stem cell (HSC) specification.
Author: Erich W. Damm
Posted: April 10, 2017, 12:00 am
Turco et al. derive long-term genetically stable organoids from normal endometrium and the decidua that recapitulate characteristics of in vivo uterine glands, respond to hormones and differentiate into secretory and ciliated endometrial cells.
Author: Margherita Y. Turco
Posted: April 10, 2017, 12:00 am
The Cdc45–MCM–GINS (CMG) complex is needed for DNA replication, but how CMG dissociates from DNA in higher eukaryotes is not clear. Sonneville et al. now characterize pathways for CMG dissociation and replication termination in worm and frog.
Author: Remi Sonneville
Posted: April 3, 2017, 12:00 am
Chatzinikolaou et al. show that the nucleotide excision repair complex ERCC1–XPF cooperates with the chromatin organizer CTCF, cohesin subunits and ATRX to facilitate the silencing of a subset of imprinted genes in the developing liver.
Author: Georgia Chatzinikolaou
Posted: April 3, 2017, 12:00 am