Bioinformatics Services

Contract research organizations (CROs) offer comprehensive functional genomics laboratory services. Such laboratory services often depends on bioinformatic analysis of the data.

As further development of functional genomics applications and associated laboratory services a number of bioinformatics software applications were developed for gene and genome sequencing applications, genetic and genomic data analysis, high through data analysis, whole-genome library screening experiments, etc. For example, Sanger sequencing has been utilized for conventional sequencing applications as well as to evaluate positive RNAi screening results, while the microarray hybridization assays are commonly used as a means of evaluating large expression data sets and negative selection screens.

These methods are relatively costly and time consuming, therefore an alternative Next Generation Sequencing (NGS) has been recently developed. Using massive parallel sequencing provide advantage both in terms of range and input library flexibility, as well as scaling up applications.

Bioinformatics Services (Company):

Forensic Bioinformatics – Link

NCBI Genbank – Link

EMBL-EBI Tools, Services, Datatases – Link

Q2 Genomic Testing and Expression Analysis – Link

Sequentix Bioinformatics Services – Link

Craic Computing – Link

BaseClear Bioinformatic Services – Link

 

News:

Nature Cell Biology - Issue - nature.com science feeds

Articles and research papers on cell division, cell structure, animal and plant cell biology and cell cycles.

The MTM1–UBQLN2–HSP complex mediates degradation of misfolded intermediate filaments in skeletal muscle

The MTM1–UBQLN2–HSP complex mediates degradation of misfolded intermediate filaments in skeletal muscle, Published online: 22 January 2018; doi:10.1038/s41556-017-0024-9

Gavriilidis et al. show that MTM1, which is mutated in X-linked centronuclear myopathy, and UBQLN2 recognize misfolded desmin and vimentin and trigger their degradation to clear misfolded intermediated filaments prior to aggregate formation.
Author: Christos Gavriilidis
Posted: January 22, 2018, 12:00 am

Affinity purification of cell-specific mitochondria from whole animals resolves patterns of genetic mosaicism

Affinity purification of cell-specific mitochondria from whole animals resolves patterns of genetic mosaicism, Published online: 22 January 2018; doi:10.1038/s41556-017-0023-x

Ahier et al. describe a method to isolate intact mitochondria from specific cells in Caenorhabditis elegans and show that the germline is more prone to propagating deleterious mitochondrial genomes than somatic lineages.
Author: Arnaud Ahier
Posted: January 22, 2018, 12:00 am

MSK1 regulates luminal cell differentiation and metastatic dormancy in ER+ breast cancer

MSK1 regulates luminal cell differentiation and metastatic dormancy in ER<sup>+</sup> breast cancer, Published online: 22 January 2018; doi:10.1038/s41556-017-0021-z

Gawrzak et al. show that MSK1 regulates bone metastatic dormancy of ER+ breast cancer. MSK1 affects histone modifications at luminal transcription factor promoters to prevent cell differentiation and bone homing.
Author: Sylwia Gawrzak
Posted: January 22, 2018, 12:00 am

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis

RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis, Published online: 22 January 2018; doi:10.1038/s41556-017-0022-y

RIP3 regulates mitochondrial metabolism. Yang et al. show that RIP3 activates the pyruvate dehydrogenase complex to enhance aerobic respiration and increase mitochondrial ROS during necroptosis, and MLKL is required for RIP3 translocation to mitochondria.
Author: Zhentao Yang
Posted: January 22, 2018, 12:00 am

Myosin-Va is required for preciliary vesicle transportation to the mother centriole during ciliogenesis

Myosin-Va is required for preciliary vesicle transportation to the mother centriole during ciliogenesis, Published online: 15 January 2018; doi:10.1038/s41556-017-0018-7

Wu et al. show that myosin-Va is needed for the initiation of primary cilia formation by transporting preciliary vesicles to the distal appendages of the mother centriole in a manner dependent on both microtubules and centrosomal actin.
Author: Chien-Ting Wu
Posted: January 15, 2018, 12:00 am

Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells

Transient Scute activation via a self-stimulatory loop directs enteroendocrine cell pair specification from self-renewing intestinal stem cells, Published online: 15 January 2018; doi:10.1038/s41556-017-0020-0

Chen et al. demonstrate that transient Scute activation induces asymmetric division in Drosophila intestinal stem cells, which generates enteroendocrine progenitor cells that divide once before differentiation to yield a pair of enteroendocrine cells.
Author: Jun Chen
Posted: January 15, 2018, 12:00 am

Segregation of mitochondrial DNA heteroplasmy through a developmental genetic bottleneck in human embryos

Segregation of mitochondrial DNA heteroplasmy through a developmental genetic bottleneck in human embryos, Published online: 15 January 2018; doi:10.1038/s41556-017-0017-8

Floros et al. show that mtDNA copy number is reduced and non-synonymous mtDNA mutations are eliminated to prevent mtDNA mutation accumulation in germ cells during human primordial germ cell development.
Author: Vasileios I. Floros
Posted: January 15, 2018, 12:00 am

EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation

EXD2 governs germ stem cell homeostasis and lifespan by promoting mitoribosome integrity and translation, Published online: 15 January 2018; doi:10.1038/s41556-017-0016-9

By analysing the exonuclease EXD2, Silva et al. find that it localizes to mitochondria, and that its loss alters metabolism by affecting mitochondrial translation and causes developmental delay and lifespan extension in flies.
Author: Joana Silva
Posted: January 15, 2018, 12:00 am