Contract research organizations (CROs) offer comprehensive functional genomics laboratory services. Such laboratory services often depends on bioinformatic analysis of the data.
As further development of functional genomics applications and associated laboratory services a number of bioinformatics software applications were developed for gene and genome sequencing applications, genetic and genomic data analysis, high through data analysis, whole-genome library screening experiments, etc. For example, Sanger sequencing has been utilized for conventional sequencing applications as well as to evaluate positive RNAi screening results, while the microarray hybridization assays are commonly used as a means of evaluating large expression data sets and negative selection screens.
These methods are relatively costly and time consuming, therefore an alternative Next Generation Sequencing (NGS) has been recently developed. Using massive parallel sequencing provide advantage both in terms of range and input library flexibility, as well as scaling up applications.
Bioinformatics Services (Company):
Forensic Bioinformatics – Link
NCBI Genbank – Link
EMBL-EBI Tools, Services, Datatases – Link
Q2 Genomic Testing and Expression Analysis – Link
Sequentix Bioinformatics Services – Link
Craic Computing – Link
BaseClear Bioinformatic Services – Link
Nature Cell Biology - Issue - nature.com science feeds
Articles and research papers on cell division, cell structure, animal and plant cell biology and cell cycles.
A transient pool of nuclear F-actin at mitotic exit controls chromatin organization
A transient pool of nuclear F-actin at mitotic exit controls chromatin organization, Published online: 13 November 2017; doi:10.1038/ncb3641Baarlink et al. identify a transient pool of nuclear F-actin, the dynamics of which are controlled by cofilin-1 that accumulates after mitosis and is important for chromatin reorganization in G1.
PARPi focus the spotlight on replication fork protection in cancer
PARPi focus the spotlight on replication fork protection in cancer, Published online: 31 October 2017; doi:10.1038/ncb3638PARP inhibitors (PARPi) kill BRCA1/2-mutated cancers, which become resistant when DNA repair functions are restored. Now, MUS81 nuclease inhibition due to EZH2 downregulation is found to restore DNA replication fork protection but not repair, leading to PARPi-resistance in mutant BRCA2 cells and patients. This challenges the DNA repair dominance in synthetic lethality.
Erratum: Super-resolution microscopy reveals that disruption of ciliary transition-zone architecture causes Joubert syndrome
Erratum: Super-resolution microscopy reveals that disruption of ciliary transition-zone architecture causes Joubert syndrome, Published online: 31 October 2017; doi:10.1038/ncb3622
Implantable synthetic organoid matrices for intestinal regeneration
Implantable synthetic organoid matrices for intestinal regeneration, Published online: 31 October 2017; doi:10.1038/ncb3635Organoids are a powerful tool to study both physiological and disease processes. A completely synthetic matrix assembled from exchangeable modular parts has been developed and not only supports proliferation of human intestinal organoids derived from pluripotent embryonic stem cells, but also augments subsequent ad vivo implantation into injured murine colon.
Reforms spell optimism for biological research in China
Reforms spell optimism for biological research in China, Published online: 31 October 2017; doi:10.1038/ncb3639A series of government reforms aim to enhance basic research efforts in China with a shift in focus from quantity to quality.
TYRP1 mRNA goes fishing for miRNAs in melanoma
<i>TYRP1</i> mRNA goes fishing for miRNAs in melanoma, Published online: 31 October 2017; doi:10.1038/ncb3637A variety of non-coding RNAs have been reported as endogenous sponges for cancer-modulating miRNAs. However, miRNA trapping by transcripts with protein-coding functions is less understood. The mRNA of TYRP1 is now found to sequester the tumour suppressor miR-16 on non-canonical miRNA response elements in melanoma, thereby promoting malignant growth.
TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at the trans-Golgi
TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at the <i>trans</i>-Golgi, Published online: 30 October 2017; doi:10.1038/ncb3627Shin et al. identify TBC1D23 as an adaptor that interacts both with golgins and endosomal WASH and is required for the delivery of endosome-derived vesicles to the trans-Golgi.
Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation
Class III phosphatidylinositol-3-OH kinase controls epithelial integrity through endosomal LKB1 regulation, Published online: 30 October 2017; doi:10.1038/ncb3631O’Farrell et al. show that class III PI3K regulates epithelial integrity through endosomal LKB1. Class III PI3K inactivation dysregulates LKB1 to alter cell polarity, and the PtdIns3P effector WDFY2 regulates LKB1.